Protein_Domain
Part:BBa_K2124003
Designed by: Brian Dempsey Group: iGEM16_Lethbridge_HS (2016-10-13)
Venom Serine Protease from Cerastes Cerastes - Thrombin analog - DsbC Disulfide shuffling
This Serine protease is a component of Cerastes Cerastes (Desert Horned Viper) venom. It recognizes Thrombin cut sites in fibrinogen and upon cleavage converts the fibrinogen to fibrin monomers. The fibrin monomers can then link and begin clot formation in mammals. This part is polycistronic and also contains DsbC which is secreted to the periplasmic space. DsbC shuffles the Serine Protease disulfide bonds for proper folding and protein activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 997
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |